| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.069198 |
| Chromosome: | mitogenome |
| Location: | 7396 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreMt.g802341 | 801491,nad2,ChrepMp05 | (1 of 1) K03879 - NADH-ubiquinone oxidoreductase chain 2 (ND2); NADH dehydrogenase subunit 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAAGCGCCAATGCTACCAATAAACATAGAGAACAAGATAAATACACCTA |
| Internal bar code: | TCTCGTGGGTAATAGCGTATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 339 |
| LEAP-Seq percent confirming: | 22.2222 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACGGCCATGTGTCTTTGG |
| Suggested primer 2: | TAGCTCAGTGGCTGGGATCT |