| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069199 |
| Chromosome: | chromosome 16 |
| Location: | 1504631 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g653000 | (1 of 1) K18404 - tudor domain-containing protein 3 (TDRD3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTCTGTTGTAGCAGGATAAGGGGGTTCCATGCCAAACCTTCCCTGATG |
| Internal bar code: | GCCTTATGTCGGCGTGGTATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 543 |
| LEAP-Seq percent confirming: | 82.2222 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTTGGAGTCGGAGCAACC |
| Suggested primer 2: | TGACCCAGCAATGACAGTGG |