Insertion junction: CLIP2.069203_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.069203
Chromosome:chromosome 12
Location:5111588
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre12.g801421 (1 of 726) IPR011009 - Protein kinase-like domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATGGTCGGGAACACCTTTCCAGTATCTTACTCGCTCACCTTGTCCTCCT
Internal bar code:ACATTTTAGCTATGCCTCCACC

Confirmation - LEAP-Seq

LEAP-Seq distance:2968
LEAP-Seq percent confirming:7.14286
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:52
LEAP-Seq n unique pos:56

Suggested primers for confirmation by PCR

Suggested primer 1:CACGCCAAACAGAGTTGTGG
Suggested primer 2:AAGCGCGCAGATTATTGCTG