| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.069216 |
| Chromosome: | chromosome 12 |
| Location: | 6090656 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g535019 | (1 of 139) IPR029787 - Nucleotide cyclase | 3'UTR | |
| Cre12.g535050 | (1 of 8) PF00211//PF01547 - Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc) // Bacterial extracellular solute-binding protein (SBP_bac_1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACATCGCAAGCATCATAAACAGTTGGCCACAAGACCTCGAGTAGGTCG |
| Internal bar code: | TCGGGAAATAACGCTTCAATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4610 |
| LEAP-Seq percent confirming: | 89.0511 |
| LEAP-Seq n confirming: | 122 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 137 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACACCGGCAAGCTTTTAA |
| Suggested primer 2: | GAGGAGTCCGTGCTGTTGAA |