| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.069222 |
| Chromosome: | chromosome 8 |
| Location: | 208532 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358553 | (1 of 1) IPR000104//IPR001810//IPR006553 - Antifreeze protein, type I // F-box domain // Leucine-rich repeat, cysteine-containing subtype | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACCGGTTGATACAACTACTCATCATGACATGTAGCTGTTCAATGCATT |
| Internal bar code: | ATCTCCCCCTGGTAGGGACCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1465 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTGTGTGTCACCCGTAC |
| Suggested primer 2: | CGCAAACTGCATCTAGACGC |