| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.069248 |
| Chromosome: | chromosome 12 |
| Location: | 4054667 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g516400 | SPL24 | Pre-mRNA splicing factor; (1 of 1) PF04146 - YT521-B-like domain (YTH) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGGACGATCCTTCGTGACCTCCCGCGCATTAGTCGCAAGCGTTAGGCT |
| Internal bar code: | GGAGTAGGGCGATTTATTTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1507 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTCCGCCTCTACGTCCTG |
| Suggested primer 2: | CATGACCATGATGCAGCAGC |