| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.069255 |
| Chromosome: | chromosome 16 |
| Location: | 4977667 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686300 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCCTGGATCCCAGCCCAGGTTGCATGCTCATGAGTGACTTGTCGGGC |
| Internal bar code: | AAGAATTGCTGGTTTCGTATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2401 |
| LEAP-Seq percent confirming: | 89.7436 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGTCGAGGACGCCCATTT |
| Suggested primer 2: | AGGTAGGAGGCAGGAGACAG |