Insertion junction: CLIP2.069269_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.069269
Chromosome:chromosome 12
Location:670692
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGCGCTACTCGCTCAGCGCCCCCGGTCCTCGTCACCAGCTGCAGCCAG
Internal bar code:TTGTGTAGTTCGGTTCTCTCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:4474
LEAP-Seq percent confirming:98.1818
LEAP-Seq n confirming:54
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:55

Suggested primers for confirmation by PCR

Suggested primer 1:CTGGATGCCACTACCGGTTT
Suggested primer 2:GGTGCTGCAAACGACCAAAT