| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.069271 |
| Chromosome: | chromosome 9 |
| Location: | 3917957 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g398067 | FKB62,ROF1 | Peptidyl-prolyl cis-trans isomerase, FKBP-type; (1 of 1) PTHR10516:SF324 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP62 | outside_mRNA |
| Cre09.g398104 | (1 of 1) K19306 - 18S rRNA (guanine1575-N7)-methyltransferase [EC:2.1.1.309] (BUD23) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGTGTGTGTGTGGGGGGGGGTGTAGATACCAGCCCAAACTTAACCGAA |
| Internal bar code: | AGTGTCTTTTGGGTTTTTTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 268 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGTGTGTTCGGTCAGAGA |
| Suggested primer 2: | GGATGTGAAAGGAGAGGGGC |