| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.069276 |
| Chromosome: | chromosome 8 |
| Location: | 443533 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358800 | (1 of 2) 5.1.1.17 - Isopenicillin-N epimerase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGGGCACGGTGGACGCGGCGGCACTCACCGCCGCACCCGCACCCGGCG |
| Internal bar code: | GAATCGATTCGTACAAGAGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 698 |
| LEAP-Seq percent confirming: | 92.5926 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGAGTTTGTTCCACACGC |
| Suggested primer 2: | GTTCAGCACTCACGTCCTCA |