Insertion junction: CLIP2.069280_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.069280
Chromosome:chromosome 7
Location:1773723
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g325723 ADK1 Adenylate kinase; (1 of 1) PTHR23359//PTHR23359:SF85 - NUCLEOTIDE KINASE // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGAAGCGGAGAAAGAAACACTGGGTTTCAACAGGGGGAACTAGATAAC
Internal bar code:ATAATCTTCACGTTTCAATTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1429
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:TATGTGCGGTTGGTGTCGAA
Suggested primer 2:CGTCCGTGTTTTAGTCCCCA