| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069319 |
| Chromosome: | chromosome 9 |
| Location: | 649005 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g404850 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATATCTTCTACTCACTGGACTAGGGTGGACTCTCCTGCAAAACTCTGAC |
| Internal bar code: | AGCGGTTTTGCTAAAAGCTACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 228 |
| LEAP-Seq percent confirming: | 1.9802 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 99 |
| LEAP-Seq n unique pos: | 101 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGAACAGCGCTTTGAGGCA |
| Suggested primer 2: | ACCCAGAAGGAGGAGCTCAT |