Insertion junction: CLIP2.069328_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.069328
Chromosome:chromosome 2
Location:5082291
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGGACACAGCAAATGACCAGAGTGGTACCCGCGTGGGCTTGGGCTTTG
Internal bar code:GACGATTGTTGCCACACAAACA

Confirmation - LEAP-Seq

LEAP-Seq distance:3949
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:52
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:52

Suggested primers for confirmation by PCR

Suggested primer 1:TACTGCACAAACGAGTCGCT
Suggested primer 2:CCGGATGACATCTCTCGGTG