| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.069339 |
| Chromosome: | chromosome 16 |
| Location: | 4301132 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g669125 | ADH13B | Dual function alcohol/acetaldehyde dehydrogenase, pseudogene 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAGTTGCTTTGCCTCAAAGCACCCATGTCCGAACAAATGTGAAAACGTT |
| Internal bar code: | GAGGGGCGGGAACATGTCACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 721 |
| LEAP-Seq percent confirming: | 57.6923 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTGAAGCTTGGATCTGGT |
| Suggested primer 2: | CGTTGCTGGCAAGATCGATG |