| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069344 |
| Chromosome: | chromosome 12 |
| Location: | 2346153 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g508000 | TIC40 | (1 of 5) IPR006636 - Heat shock chaperonin-binding; Putative chloroplast enveloppe protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCACAGGAATTCCCTGGCACTAATTTCTTCTCCCCTCTCTAGTTAGTT |
| Internal bar code: | GATGGTCGATGAAGAACCAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 889 |
| LEAP-Seq percent confirming: | 26.6667 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCTCCTCCTTGTAGGGAG |
| Suggested primer 2: | GATTCGTCCAGAGCACAGCT |