Insertion junction: CLIP2.069346_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.069346
Chromosome:chromosome 16
Location:4009887
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g689950 (1 of 1) PF00168//PF08372 - C2 domain (C2) // Plant phosphoribosyltransferase C-terminal (PRT_C) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTCGAATTTACTACTGCCGGCGTGAGGCGGCGGCAGCAGCTGCTGGCAT
Internal bar code:CATCCCGGGGTAGTTATTCTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:890
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GGCTTTACGCGCTAAGAAGC
Suggested primer 2:CCACCACAGCGAAAAGTGTG