| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069350 |
| Chromosome: | chromosome 2 |
| Location: | 4481430 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g106550 | (1 of 1) K12839 - survival of motor neuron-related-splicing factor 30 (SMNDC1, SPF30) | 3'UTR | |
| Cre02.g800227 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTTCGTAAGATACGAGCGCTGGACGCGAACCCATGTAAGTGCTCACGC |
| Internal bar code: | GACACTCACGATGCCCGTCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1733 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCATCAAGAAGGGCAGCA |
| Suggested primer 2: | AGCGCTCGTATCTTCCTTCG |