| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.069358 |
| Chromosome: | chromosome 13 |
| Location: | 2817168 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g801539 | (1 of 1) PTHR14140:SF27 - PROTEIN SHISA-5 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGAGAACCCTGCGCGGGGGTGCCCCGGCGGTACCCTCTGCCATCCTTA |
| Internal bar code: | ATGACTATTCAGAGATGTGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5217 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 88 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCATTTCTCAGGGGTCACC |
| Suggested primer 2: | CCGGAATGGTTTCTCTGCCT |