| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069360 |
| Chromosome: | chromosome 13 |
| Location: | 5105787 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g606850 | (1 of 1) K14548 - U3 small nucleolar RNA-associated protein 4 (UTP4, CIRH1A) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATGTGCTGGCAACTGGCAATGCAGTAGCTGTCTGCATGCGTCCGGAGT |
| Internal bar code: | TTTAGCCACATTGCAATGGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4893 |
| LEAP-Seq percent confirming: | 99.2064 |
| LEAP-Seq n confirming: | 125 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 126 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAACGAGCGCTGTCACACT |
| Suggested primer 2: | TACCGTCTTGTGGTGAACGG |