| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069370 |
| Chromosome: | chromosome 14 |
| Location: | 1420274 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g617100 | SRS9,SRS12 | Serine/arginine-rich pre-mRNA splicing factor; (1 of 89) PF00076 - RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain) (RRM_1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGTTGGCTCAGTACTTCACTCCTCCGACTCCGGATTCAAGAAATTTT |
| Internal bar code: | TACGGACATAAAAGGGTTTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6233 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTATCGCGCACAACCCATTG |
| Suggested primer 2: | AACGACCCTACATTGCACGT |