| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.069381 |
| Chromosome: | chromosome 9 |
| Location: | 5213918 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g406950 | (1 of 5) IPR000104//IPR016024 - Antifreeze protein, type I // Armadillo-type fold | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGCGGCGGTCGCGGTGCGCGGTGCGGCGACGGCCGCAGAGAAAGGGGT |
| Internal bar code: | TTTTGGGGAACCGTTAGCTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1594 |
| LEAP-Seq percent confirming: | 92.5926 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGGGCCAGGAGCAATAAA |
| Suggested primer 2: | TAACACTCGCACACAGGGAC |