Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | + |
Strain: | CLIP2.069382 |
Chromosome: | chromosome 9 |
Location: | 3263073 |
Confidence (%): | 40 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g394954 | (1 of 1) K03975 - membrane-associated protein (dedA) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTTCGGCATGCCGCCTCCCACCAGGTACCGCGCGCCTGCCGCCCGTCT |
Internal bar code: | GGGCGGGGATCGTGTGTATCGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1105 |
LEAP-Seq percent confirming: | 28.5714 |
LEAP-Seq n confirming: | 2 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCAAGCCCAACTTTGCATGA |
Suggested primer 2: | AGGCACAGTGATGAACCCTG |