Insertion junction: CLIP2.069383_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.069383
Chromosome:chromosome 14
Location:1788120
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g801621 (1 of 14) IPR006626//IPR011050 - Parallel beta-helix repeat // Pectin lyase fold/virulence factor 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTGCCACGCACGCTGCGGGTGGCGCCGTCAACATCCAGACCTCTCGTT
Internal bar code:GGGCATTTCATTTGACCAATCA

Confirmation - LEAP-Seq

LEAP-Seq distance:2821
LEAP-Seq percent confirming:85.7143
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:ATCGGCGCTGTTGTTGTAGA
Suggested primer 2:CCTTGCCGTATGACACCTGT