Insertion junction: CLIP2.069408_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.069408
Chromosome:chromosome 8
Location:3668328
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g380000 UMM7 UbiE/COQ5 methyltransferase family protein; (1 of 1) PTHR10108//PTHR10108:SF894//PTHR23150//PTHR23150:SF24 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED // SULFATASE MODIFYING FACTOR 1, 2 // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACGCGTGTACTCGTCACCCCAATTAACCGTTCTACTACCGCACTCGG
Internal bar code:AAACTCTATGGTCTAACAGAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:3551
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:78
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:78

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGGACATCAGCAAGACCT
Suggested primer 2:CTGTCGCTTCCCCACTTGAT