| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.069409 |
| Chromosome: | chromosome 6 |
| Location: | 1501301 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g260600 | PPP22 | Phosphoprotein phosphatase 2A regulatory subunit related; (1 of 2) K11583 - serine/threonine-protein phosphatase 2A regulatory subunit B'' (PPP2R3) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGAGTGTCCGAGGGTGGCAGGGATGTCAGGCGGGCCATCCAGCGCATC |
| Internal bar code: | GCTGTGTGTTTTGCAGGATGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3608 |
| LEAP-Seq percent confirming: | 92.7536 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTTGTCTGGGCACAAGGC |
| Suggested primer 2: | GCGCATGGATAGTTTGCTGG |