| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069413 |
| Chromosome: | chromosome 5 |
| Location: | 2510443 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g239200 | FAP275 | Flagellar Associated Protein 275 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTATTAATGATGAAATGATGCAAAGTGAAAAACCGAAAGCTCTCTGACT |
| Internal bar code: | GCCTTCGGAATGATAGCTACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3737 |
| LEAP-Seq percent confirming: | 89.7727 |
| LEAP-Seq n confirming: | 79 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 88 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTTGCTATCCTGCACCT |
| Suggested primer 2: | GTTAGCAGGTGGAGCATGGT |