| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.069424 |
| Chromosome: | chromosome 10 |
| Location: | 1863362 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g431100 | OPR126 | OctotricoPeptide Repeat protein 126 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCAGCCAGGTTGAGGCTTGAGATAGATTTTCGCTCGTGGAGGGGCTAT |
| Internal bar code: | TACAGTTTTGGAATGACTCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2553 |
| LEAP-Seq percent confirming: | 86.6667 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTTCCAGTTGTCCCTTCC |
| Suggested primer 2: | GCTGATGATTGGCAGGCATG |