Insertion junction: CLIP2.069428_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.069428
Chromosome:chromosome 10
Location:3805694
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g445650 SMC3 (1 of 1) K06669 - structural maintenance of chromosome 3 (chondroitin sulfate proteoglycan 6) (SMC3, CSPG6); Structural Maintenance of Chromosomes protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACGACCGACCCCTCAACTTTGGCGGCCCAGAACTTCGTTCCTCATCAGA
Internal bar code:GGGAGCGACCTGTAGGTGGGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:4746
LEAP-Seq percent confirming:92.3664
LEAP-Seq n confirming:121
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:131

Suggested primers for confirmation by PCR

Suggested primer 1:CGGGTGGAGATGACGATGAG
Suggested primer 2:TCCCGTAAGCCTAGACAGCT