Insertion junction: CLIP2.069429_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.069429
Chromosome:chromosome 6
Location:8629743
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre06.g309900 MST2 Similar to Mastigoneme protein; (1 of 4) IPR009030//IPR011641 - Insulin-like growth factor binding protein, N-terminal // Tyrosine-protein kinase ephrin type A/B receptor-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCCCAGAGCCTCACCTACAACCTGGCCAACGAGACGGCGCCCAACACG
Internal bar code:AGCGTATATTGTCGTAAATTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:778
LEAP-Seq percent confirming:25.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTTTGGTTGTCGTCACCG
Suggested primer 2:GAGTACACGGTGCTTCCCAA