| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.069441 |
| Chromosome: | chromosome 13 |
| Location: | 1383214 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g571850 | APC10 | Anaphase promoting complex subunit 10; (1 of 1) K03357 - anaphase-promoting complex subunit 10 (APC10, DOC1) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCAAACGGACCAGTGCATGCGGGTGCGTCTGCATCGCGCAGGCGTTTC |
| Internal bar code: | GGAGCGTTTAGTGGGAAGCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1597 |
| LEAP-Seq percent confirming: | 96.875 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTGGTCACCTGGCGTAAA |
| Suggested primer 2: | ACATACGACAGGTGCGTGTT |