| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.069442 |
| Chromosome: | chromosome 12 |
| Location: | 7852360 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g553750 | SPL26 | Pre-mRNA splicing factor; (1 of 2) K13095 - splicing factor 1 (SF1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAAACTGTCCACGCGTTTTGCCAAAGTGGTTTAATTCATGCGTGTGTCT |
| Internal bar code: | GAAGCGGACGTTGATTGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1472 |
| LEAP-Seq percent confirming: | 80.4878 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTCATTGGAGTTCGGGA |
| Suggested primer 2: | AAGTAAGGCACAAGGACGGG |