| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.069444 |
| Chromosome: | chromosome 2 |
| Location: | 7903668 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g145628 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTCAGACAGTGCGGCATGACGGCACAACGATAGAGTGTCACTGAATGG |
| Internal bar code: | CGGGCTTCGAAGCCAAGGGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2521 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAACAACAACGTGTGCCCC |
| Suggested primer 2: | TCCAGCTCAGCATTGACAGG |