| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.069456 |
| Chromosome: | chromosome 13 |
| Location: | 1760483 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g574650 | (1 of 2) 3.6.1.62 - 5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase / M(7)GpppN-mRNA hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTATTGCAGATTGGGCTCTTACACGTCACCGCGAAGCAGGCCACTTCA |
| Internal bar code: | TTTTCATGGGTGTTCGTTGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1827 |
| LEAP-Seq percent confirming: | 97.7011 |
| LEAP-Seq n confirming: | 85 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 87 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCACAGGAGCTGAAAGAG |
| Suggested primer 2: | ACACGTTTTTGGGCGGATTG |