| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069474 |
| Chromosome: | chromosome 5 |
| Location: | 110533 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g800507 | (1 of 10) 3.1.4.46 - Glycerophosphodiester phosphodiesterase / Glycerophosphoryl diester phosphodiesterase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGTGAGCGAGCCCGAGAATCTCACATGCCTCGTCCGAGCAGGAACTCG |
| Internal bar code: | AGGCTGAACCGGGCAGAAGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 802 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACACAAGCAGCGGGAAGTT |
| Suggested primer 2: | ATAGGACCCAGCTCGTCACT |