Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.069480 |
Chromosome: | chromosome 10 |
Location: | 5225953 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g456100 | FAP142,POC21,AGG3 | (1 of 4) K03809 - NAD(P)H dehydrogenase (quinone) (wrbA); Aggregation 3 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATTGAAGCACATGGCTCTCGGCCGTTGCAATCACCGCCTGCCGTACGG |
Internal bar code: | TCCCCTATATTTACTGGTTGCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2866 |
LEAP-Seq percent confirming: | 76.4706 |
LEAP-Seq n confirming: | 52 |
LEAP-Seq n nonconfirming: | 16 |
LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGGTAGCATCATGTTTGGCG |
Suggested primer 2: | CCATGGCAAAGCGTGATAGC |