Insertion junction: CLIP2.069480_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.069480
Chromosome:chromosome 10
Location:5225954
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g456100 FAP142,POC21,AGG3 (1 of 4) K03809 - NAD(P)H dehydrogenase (quinone) (wrbA); Aggregation 3 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTTAGCTGTCGTGGTTGTCCGTGCATGCACGCGGTTTTGTGCAACAGTG
Internal bar code:TCCCCTATATTTACTGGTTGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:694
LEAP-Seq percent confirming:93.5484
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:CCATGGCAAAGCGTGATAGC
Suggested primer 2:CGGTAGCATCATGTTTGGCG