Insertion junction: CLIP2.069496_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.069496
Chromosome:chromosome 16
Location:607098
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g692100 outside_mRNA
Cre16.g692150 GT90-29,GT90F29 (1 of 11) 2.4.2.26 - Protein xylosyltransferase / Uridine diphosphoxylose-protein xylosyltransferase; GT90 family protein 29 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCATACTCACAGAGACAACATGTAAATTGCATCTGTAATTGATTTGCGCC
Internal bar code:CTACAAATCTTCGGCGTACGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:2121
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:TGGTAGGTGGCGTGTTCATC
Suggested primer 2:GCAGGTGGCGAACATTTTGT