| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.069499 |
| Chromosome: | chromosome 10 |
| Location: | 2704942 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g437700 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGAAGTAAGCATGCGCACTACTCGGTCGGCGGAAGTTTCATGCAAATT |
| Internal bar code: | CTGCCGGCGTCGCACTTGACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2712 |
| LEAP-Seq percent confirming: | 46.875 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTTGCTGCTGCTGTCTCT |
| Suggested primer 2: | GCTTGCTGGAGATGTGACCT |