Insertion junction: CLIP2.069510_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.069510
Chromosome:chromosome 14
Location:3414460
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g630050 (1 of 6) PTHR13339//PTHR13339:SF0 - COP9 SIGNALOSOME COMPLEX SUBUNIT 8 // COP9 SIGNALOSOME COMPLEX SUBUNIT 8 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACGCCCCCCCACGCATGAGGTACTTCTTAAGCCACCGGCCCAGCCGCC
Internal bar code:GGAACTGTTCGCGGGATACAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1665
LEAP-Seq percent confirming:86.6667
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGATGCTGTCCCTGGTTC
Suggested primer 2:CTAGGCAGCGAGAGAAGAGC