| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.069511 |
| Chromosome: | chromosome 12 |
| Location: | 3832544 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g514950 | CGLD17,HEL53 | (1 of 1) PTHR12873//PTHR12873:SF0 - T7-LIKE MITOCHONDRIAL DNA HELICASE // TWINKLE PROTEIN, MITOCHONDRIAL; Conserved in the Green Lineage and Diatoms | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGATCTGGATTCGGTCGCGGCGTCCCGGGACGTGGCTGGACACATTATG |
| Internal bar code: | TGAAGGTCGGTGGGAGTGGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2427 |
| LEAP-Seq percent confirming: | 97.7778 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGTGAACTCATGCGCATC |
| Suggested primer 2: | GCAGTTGGGTCGTAGAAGCT |