| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.069524 |
| Chromosome: | chromosome 7 |
| Location: | 4586406 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g344172 | 5'UTR | ||
| lncRNA_TCONS_00101329 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAACCCGAGTGCAAATTTGCACCCAATTTGCCTTATTTGCGCGTTCTGA |
| Internal bar code: | GATTGCGACGGTCATCGGGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3392 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACATTGTACCGGTGGTGT |
| Suggested primer 2: | CGTGCCTCGTCCTGTAAGTT |