| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.069549 |
| Chromosome: | chromosome 11 |
| Location: | 3685344 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g478626 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCACAAGCGGACACACACAGCACCGCACACGCACACGCACACACACGC |
| Internal bar code: | GTTAAGTTCAGTGAAGTCACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 574 |
| LEAP-Seq percent confirming: | 59.5745 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACCTCAACCGCTTCTCT |
| Suggested primer 2: | CACGTGTCAGCTTGTGTGTG |