| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.069550 |
| Chromosome: | chromosome 17 |
| Location: | 5749193 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g740850 | (1 of 781) IPR000104 - Antifreeze protein, type I | 5'UTR | |
| Cre17.g740900 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGAGGGAACGCCCAGCACGCATTCGCAGCCACCCTCCACAACGCTTCT |
| Internal bar code: | AGAATAGTGGACTTCAGTCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1574 |
| LEAP-Seq percent confirming: | 22.2222 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGGGTATCTGTTGTGCT |
| Suggested primer 2: | ACATGTCACACGTAGGCCTG |