| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.069553 |
| Chromosome: | chromosome 4 |
| Location: | 1615832 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213000 | MAE12 | (1 of 5) PTHR11206:SF94 - DNA-DAMAGE-INDUCIBLE PROTEIN F; MATE efflux family protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGTGGTGGTGACGTTGGGCGTGGCAGCGGCGGCGGCATCTGCGGCGGC |
| Internal bar code: | CCCAATGGTGTGTCAGTTAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2123 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTGTTGGGTTGTCAGCG |
| Suggested primer 2: | TTTGTGCGCTGTAACACACG |