| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.069558 |
| Chromosome: | chromosome 9 |
| Location: | 2678287 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391900 | TRXH1,TRXH,TRXh1,Chh1,TRX5 | (1 of 9) K03671 - thioredoxin 1 (trxA); Thioredoxin h1, cytosolic | 3'UTR |
| Cre09.g801021 | intron | ||
| lncRNA_TCONS_00129093 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGGCCGATGTCCGTCACCTCAGCTCATCACGGGGGCACGATCACCTGA |
| Internal bar code: | CGTTCTCGCTCGTTCTCAAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 109 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAAGCGAGGAAAGGGTTG |
| Suggested primer 2: | GTTACGGTCGTGAAGTGGGT |