| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.069580 |
| Chromosome: | chromosome 14 |
| Location: | 1859174 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619850 | ELG36 | (1 of 2) 2.7.7.52 - RNA uridylyltransferase / TUT; Exostosin-like membrane protein, a heparan sulfate glycosyltransferase | 5'UTR |
| Cre14.g619854 | ELG35 | (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase; Exostosin-like glycosyltransferase 35 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTCAGCAAACAATATGGCAATTAGGATTGAGCACATCGATGAAGACCT |
| Internal bar code: | GCTTCTCGTGATATGAATGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2343 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGGAGTGCTGGGTAAA |
| Suggested primer 2: | GACAAAGGTGCCAGCAATCG |