Insertion junction: CLIP2.069596_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.069596
Chromosome:chromosome 6
Location:5285460
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g284500 RPH1,RNPH1 3'-5' Exoribonuclease PH component of the Exosome; (1 of 1) K11600 - exosome complex component RRP41 (RRP41, EXOSC4, SKI6) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGACAGGTTCCCTGGAAGGCTTCGACTTACATGTTGTTCGAGAGGTGTTG
Internal bar code:CGTTGACTAATTGTCTGTCGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:90
LEAP-Seq percent confirming:9.09091
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GGTATTCTCCTCCATGGGCG
Suggested primer 2:CTCATGATGGTGCCACAAGC