| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.069600 |
| Chromosome: | chromosome 10 |
| Location: | 1213301 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g426250 | (1 of 1) K14800 - pre-rRNA-processing protein TSR2 (TSR2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTGCGGATGGATGTATGCGCGTGGTGGTTTGGTGGTTCCGGAGTTGGG |
| Internal bar code: | TCATTAGTTACGTAGGTTATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1971 |
| LEAP-Seq percent confirming: | 82.9268 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTATGGTGCGATGCGTAGA |
| Suggested primer 2: | GCTGTGACGTGTGTCCAAAC |