Insertion junction: CLIP2.069602_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.069602
Chromosome:chromosome 6
Location:1537384
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g260850 NOP14 (1 of 1) K14766 - nucleolar protein 14 (NOP14, UTP2); Nucleolar protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTCATGCAAGACCTGTACTGTACAATTTTCGTGTCCCCAACGCTTGCGC
Internal bar code:CATGCGAACTCTACTTCAGTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:189
LEAP-Seq percent confirming:27.2727
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGGGCAATGCGTATCATG
Suggested primer 2:CGCGAGCCTTTACACCATTG