Insertion junction: CLIP2.069613_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.069613
Chromosome:chromosome 2
Location:890515
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g078950 MMP17 Matrix metalloproteinase; (1 of 40) 3.4.24.38 - Gametolysin / Lysin 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGAAGCAAGGTCCCACCGTGTCTTGTACATGCTCGTTCGGCTCCACCAC
Internal bar code:AGCAGGGGTGAGCGAGCAGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:3786
LEAP-Seq percent confirming:74.1573
LEAP-Seq n confirming:66
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:89

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTTGATCGCATGTGTAGC
Suggested primer 2:GCAAGAATAGAAGCGCGGTG